Kadar Karbohidrat dan Penapisan Senyawa Aktif dari Pleurotus pulmonarius yang Diekstraksi Menggunakan Metode Microwave Assisted Extraction (MAE) dengan Beberapa Perlakuan Suhu

The majority of carbohydrates found in edible mushroom are the form polysaccharides, specifically beta-glucans. These beta-glucans have been reported as anticancer, antioxidant, and immunomodulator. This study aimed to investigate potential P. pulmonarius functional food by comparing carbohidrate content (total carbohydrate, beta-glucans, reducing sugars) extracted using microwave assisted extraction (MAE) at different temperatures: 60oC, 80oC 100oC. Moreover, active compound screening was also conducted. MAE chosen method due its short time. phenol-sulfiric acid used measure total carbohydrates, while beta-glucan measured Megazyme Beta-Glucan Kit. Reducing sugar DNS (3,5-Di Nitro Salisilic Acid) performed qualitative method. results showed that highest were obtained 60oC (75,089±0,012 mg/mL 32,356±0,006% w/w respectively) (0,216±0,009 mg/mL). Active revealed presence flavonoids, saponins, triterpenoids each sample. findings suggest has since rich compounds. use is more effective extract compared with